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KMID : 1005220130280030207
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Journal of Embryo Transfer
2013 Volume.28 No. 3 p.207 ~ p.213
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A Parentage Test using Indel, Microsatellite Markers and Genotypes of MC1R in the Jeju Black Cattle Population
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Han Sang-Hyun
Cho Sang-Rae
Cho In-Cheol
Cho Won-Mo
Kim Sang-Geum
Yang Sung-Nyun
Kang Yong-Jun
Park Yong-Sang
Kim Young-Hoon
Park Se-Pill
Kim Eun-Young
Lee Sung-Soo
Ko Moon-Suck
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Abstract
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This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of 4.1202×10-4 than those previously recomm- ended by International Society of Animal Genetics (ISAG) of 5.000×10-4 for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of 2.679×10-5 . Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.
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KEYWORD
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molecular marker , parentage , microsatellite , indel , Jeju Black cattle
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